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[Primary selection method for microorganism producers of organic acids]
Mikrobiologiia
|March 1, 1980
Summary
A novel method using bromocresol green indicator media efficiently identifies organic acid-producing microorganisms. This technique successfully isolated 49 yeast cultures, with 38 producing significant citric acid from n-paraffins.
Area of Science:
- Microbiology
- Biochemistry
Context:
- Efficient screening of microorganisms for valuable metabolite production is crucial in biotechnology.
- Traditional methods for identifying organic acid producers can be time-consuming and labor-intensive.
Purpose:
- To develop and validate a rapid diagnostic method for primary selection of microorganisms capable of producing organic acids.
- To identify yeast cultures that can accumulate citric acid when utilizing n-paraffins as a carbon source.
Summary:
- A new method utilizes diagnostic media containing the indicator bromocresol green for the primary selection of organic acid-producing microorganisms.
- Testing various yeast fungi revealed 49 cultures producing organic acids.
- Further analysis showed that 38 of these cultures accumulated 5.0 to 30.0 mg% citric acid when cultured in a medium containing n-paraffins.
Impact:
- This method offers a faster and more effective approach for identifying promising microbial candidates for organic acid production.
- Facilitates the discovery of new yeast strains for industrial applications, particularly in citric acid biosynthesis.
- Contributes to the advancement of microbial biotechnology and sustainable chemical production.