[Biphasic energy-dependent potassium ion absorption by Escherichia coli cells]
Related Experiment Video
Updated: Oct 24, 2026

Recapitulation of an Ion Channel IV Curve Using Frequency Components
Published on: February 8, 2011
Abstract:
The E. coli cells remove acid and accumulate potassium ions in two phases in the presence of glucose. The first phase (5-7 min) takes place only with an increase of external osmolarity; the second phase begins after 20 min and is not sensitive to alteration of the osmotic pressure. Potassium efflux occurs between two phases of K+-accumulation in alkaline media. Replacement of glucose by lactate results in a decrease of the first phase and abolition of the second one. The initial rate of K+-uptake for the first phase is a saturation curve in the range of 6 mM of external potassium concentration. These data indicate that the osmosensitive system of K+-uptake is connected with the first phase.
More Related Videos
12:48Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays
Published on: February 19, 2013
08:11Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
Published on: November 11, 2022
Related Concept Videos
Primary Active Transport
Ion Channels
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Primary Active Transport
ATP Driven Pumps I: An Overview
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
Resting Potential Decay
At rest, the K+ is the main ion that moves across the membrane through...
Action Potential: Phases of Stimulation
Resting Phase:
In this phase, the cell's membrane is at its resting potential, typically around -70 millivolts (mV) for neurons. Inside the cell, there is a higher concentration of potassium ions (K+) and a lower concentration of sodium ions (Na+). Voltage-gated sodium channels are closed, and...