Serological examinations with TPHA antigen prepared from T. pallidum Budapest strain

The TPHA test is performed with sheep erythrocytes treated with formalin and tannin solution and conjugated with fragments of pathogenic Treponema pallidum. Absorption media was prepared by adding normal rabbit serum, powdered rabbit testicle, sheep and cow stroma and sonicated Reiter treponeme. The reagents prepared in our laboratory proved to be specific and sensitive enough for routine use. It can be confirmed that the TPHA reaction is a good confirmatory test in the serodiagnosis of syphilis. Further studies are necessary to explain the divergent results between TPHA and TPIT. We plan to carry out detailed investigation. Analysis of patients data in a large population after the problem of storage of our reagents over extended periods has been resolved.