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Characterization of mouse thoracic duct B lymphocytes. I. Evidence of functional heterogeneity
Thoracic duct lymphocytes (TDL) from C3H/Tif and BALB/c mice were studied for their in vitro reactivity to the B cell mitogens lipopolysaccharide (LPS) and lipoprotein (LP). Roughly 4% and 10% of the surface immunoglobulin (Ig)-positive cells in these populations could be stimulated by LPS and LP, respectively, to generate clones of IgM-secreting cells. Among LPS-reactive B cells, roughly 30% developed into clones which also produced IgG3 or IgG2, while only a very small fraction (1-2%) of all precursors could give rise to clones secreting IgG1 and IgA. Freshly collected TDL from some batches of C3H/HeJ mice displayed a high proportion of Ig-containing B cell blasts (5-10%), which did not secrete enough Ig to be detected as plaque-forming cells (PFC). These cells, however, under appropriate culture conditions and stimulated by LP (but not by Nocardia mitogen), differentiated to PFC of the various Ig classes without dividing.
Thoracic duct lymphocytes (TDL) from C3H/Tif and BALB/c mice were studied for their in vitro reactivity to the B cell mitogens lipopolysaccharide (LPS) and lipoprotein (LP). Roughly 4% and 10% of the surface immunoglobulin (Ig)-positive cells in these populations could be stimulated by LPS and LP, respectively, to generate clones of IgM-secreting cells. Among LPS-reactive B cells, roughly 30% developed into clones which also produced IgG3 or IgG2, while only a very small fraction (1-2%) of all precursors could give rise to clones secreting IgG1 and IgA. Freshly collected TDL from some batches of C3H/HeJ mice displayed a high proportion of Ig-containing B cell blasts (5-10%), which did not secrete enough Ig to be detected as plaque-forming cells (PFC). These cells, however, under appropriate culture conditions and stimulated by LP (but not by Nocardia mitogen), differentiated to PFC of the various Ig classes without dividing.