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Related Experiment Videos

Yeast viral RNA polymerase is a transcriptase

J Bruenn, L Bobek, V Brennan

    Nucleic Acids Research
    |July 11, 1980
    PubMed
    Summary
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    This study reveals that the yeast virus ScV-L contains a capsid-associated RNA polymerase. This enzyme acts as a transcriptase, producing uncapped messenger RNA for the ScV-Pl protein.

    Area of Science:

    • Virology
    • Molecular Biology
    • Yeast Genetics

    Background:

    • ScV-L is a double-stranded RNA virus found in yeast.
    • Its genome (L) encodes a major capsid protein (ScV-Pl).
    • The virus possesses a capsid-associated RNA polymerase activity.

    Purpose of the Study:

    • To characterize the function of the ScV-L capsid-associated RNA polymerase.
    • To determine the nature of the RNA synthesized by the polymerase.
    • To identify the specific RNA product synthesized in vitro.

    Main Methods:

    • In vitro transcription assays using purified ScV-L particles.
    • Analysis of synthesized RNA products by gel electrophoresis and sequencing.
    • Identification of the mRNA for ScV-Pl.

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    Main Results:

    • The capsid-associated RNA polymerase exhibits transcriptase activity.
    • The polymerase synthesizes predominantly single-stranded RNA in vitro.
    • A key product is the uncapped messenger RNA (mRNA) for ScV-Pl.

    Conclusions:

    • The ScV-L RNA polymerase functions as a transcriptase.
    • This enzyme produces uncapped mRNA essential for viral protein synthesis.
    • Understanding this viral replication mechanism provides insights into RNA virus biology.