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Hemopoietic bone culture in diffusion chambers

A Faradji, J P Bergerat, F Oberling

    Biomedicine / [Publiee Pour L'A.A.I.C.I.G.]
    |May 1, 1980
    PubMed
    Summary
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    Fibroblast-like cells found in rat femur bone marrow support the bone marrow microenvironment. These cells, originating from bone, resemble those seen in bone and marrow cultures.

    Area of Science:

    • Cell Biology
    • Hematology
    • Bone Biology

    Background:

    • Cellular components within bone marrow cavities are crucial for the bone marrow microenvironment.
    • Understanding the origin and characteristics of these cells is essential for comprehending marrow function.

    Purpose of the Study:

    • To identify and characterize fibroblast-like cells within the bone marrow microenvironment.
    • To determine the osseous origin of these cellular elements.
    • To compare in vivo cultivated cells with those from in vitro bone and marrow cultures.

    Main Methods:

    • Cultivation of diaphyseal fragments from rat femurs in vivo using diffusion chambers.
    • Histological examination of diffusion chamber membranes.
    • Study of sexual chromatin to ascertain osseous origin of fibroblast-like cells.

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    Main Results:

    • Histological analysis revealed the presence of fibroblast-like cells on the diffusion chamber membranes.
    • Sexual chromatin studies confirmed the osseous origin of these identified cells.
    • Morphological similarities were observed between these in vivo derived cells and those from in vitro cultures of bone or bone marrow.

    Conclusions:

    • Fibroblast-like cells derived from bone are present in the bone marrow microenvironment.
    • These cells play a role in the bone marrow microenvironment.
    • The findings suggest a link between bone-derived cells and the hematopoietic microenvironment, supported by morphological similarities with in vitro cultured cells.