An improved method for the detection of human T lymphocytes in tissue sections

The technique of haemadsorption to tissue sections for the detection of T lymphocytes is described in detail. The modification using the closed chamber technique is convenient, sensitive and reproducible. In principle, it parallels the rosette technique using blood lymphocytes in suspensions and AET-treated sheep erythrocytes. Storage of tissue at -25 degrees C did not reduce the activity of the E-receptors in the tissue. The E-receptors were blocked by incubation of the tissue sections with an anti-T lymphocyte serum.