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Spironolactone bodies. An immunoperoxidase study with biochemical correlation
American Journal of Clinical Pathology
|January 1, 1981
Summary
Aldosterone and cortisol are difficult to detect in adrenal tissues due to processing. However, aldosterone was found in spironolactone bodies, suggesting unusual steroid binding within these structures.
Area of Science:
- Endocrinology
- Histopathology
- Steroid Biochemistry
Background:
- Routine tissue processing often leads to the solubilization of steroid hormones.
- The localization of aldosterone and cortisol in adrenal tissues using immunoperoxidase techniques is challenging.
Purpose of the Study:
- To investigate the localization of aldosterone and cortisol in formalin-fixed paraffin-embedded adrenal glands and tumors.
- To determine the nature and origin of spironolactone bodies in relation to steroid storage.
Main Methods:
- Immunoperoxidase staining using antialdosterone and anticortisol antibodies.
- Examination of formalin-fixed paraffin-embedded adrenal gland and tumor tissues.
- Analysis of spironolactone bodies for the presence of steroid hormones.
Main Results:
- Cytoplasmic staining for aldosterone and cortisol was not observed in adrenal cortical cells.
- Spironolactone bodies demonstrated positive reactivity with antialdosterone antibody.
- Aldosterone was detected within the concentric laminations of spironolactone bodies, indicating it is not an artifact.
Conclusions:
- Spironolactone bodies likely originate from the endoplasmic reticulum, which can store steroids.
- Aldosterone appears to be bound in an unusual form within spironolactone bodies.
- These findings suggest that aldosterone and possibly cortisol-like substances can be trapped during spironolactone body formation.