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Updated: Aug 17, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
IgM-rheumatoid factors cross-reactive with IgG and a cell nuclear antigen: immunopathological implications?
Abstract:
The cross-reaction of IgM-rheumatoid factors (IgM-RF) with a cell nuclear antigen has been further investigated by immunofluorescence procedures. This reaction appears to be optimal at pH 8-9, and fails to occur at pH 6.5. No evidence was obtained by immunofluorescence that IgM-RF bound to the cell nuclear antigen fixes complement. It would appear that the nuclear reactivity of IgM-RF may be of limited immunopathological significance, though this reaction is of note in understanding the behavioural nature of rheumatoid factors.
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