Detection of antibody-coated bacteria in the bile in experimental cholecystitis

Detection of antibody-coated bacteria (A C B) in the bile sediment was tried in experimental cholecystitis which were produced in male rabbits by inoculation of mixture of E. coli and 5% sodium desoxycholate. A C B in the bile sediment was demonstrated in all cases by the direct immunofluorescent antibody technique. All cases showed positive cultures and all these isolates were the same strain as inoculated.