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[Demonstration of enteropathogenic E. coli by the ligated intestinal segment technic]
Veterinarno-Meditsinski Nauki
|January 1, 1980
Summary
A new method using ligated intestinal segments identified enteropathogenic Escherichia coli (E. coli) strains in calves. This technique revealed that enteropathogenic E. coli cause intestinal dilation due to enterotoxin production.
Area of Science:
- Veterinary Microbiology
- Animal Pathology
Background:
- Enteropathogenic Escherichia coli (E. coli) is a significant cause of calf enteritis.
- Accurate identification of pathogenic E. coli strains is crucial for effective disease management.
Purpose of the Study:
- To establish a reliable method for determining the enteropathogenic character of E. coli strains isolated from calves with enteritis.
- To identify the prevalence of enteropathogenic E. coli serotypes in affected calves.
Main Methods:
- Utilized the ligated intestinal segment technique in calves to assess E. coli pathogenicity.
- Observed intestinal segment dilation, exudate formation, and edema as indicators of enteropathogenicity.
- Defined a strain as enteropathogenic if it induced dilation in two tested calves.
Main Results:
- Enteropathogenic E. coli strains induced significant dilation in ligated intestinal segments, linked to enterotoxin production.
- Non-enteropathogenic strains did not provoke this reaction.
- Out of 272 E. coli strains, 43 (15.8%) were identified as enteropathogenic.
- Enteropathogenic strains most frequently belonged to serotypes O8, O101, and O9.
Conclusions:
- The ligated intestinal segment technique is effective for identifying enteropathogenic E. coli in calves.
- Enteropathogenic E. coli, particularly serotypes O8, O101, and O9, are prevalent causes of calf enteritis.