[Production of a high-titer agglutinating Bordetella bronchiseptica serum]

For a serological identification of Bordetella Bronchiseptica, eliminated from swine ill with atrophic rhinitis, was obtained an agglutinated antiserum. For the purpose rabbits were innoculated 1. w. 2.5-3 kg) and living cultures were used after intravenous injection. For the obtention of a bacterial suspension Müller-Hinton's medium was used. Three series of antiserum were produced against Bordetella Bronchiseptica. The first series was obtained after straining the bacterial strain into the medium of McGengou and Müller-Hinton agar and had a high titre 1:640-:1280. The second series was obtained after straining the strain through egg embryos and had a titre 1:5000-:10,000. By straining the strain through egg embryos and young white mice, an antiserum was obtained having the titre 1:20,000-1:40,000.