Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Energy source for lithium efflux in yeast

A Rodríguez-Navarro, E D Sancho, C Pérez-Lloveres

    Biochimica Et Biophysica Acta
    |January 8, 1981
    PubMed
    Summary

    Lithium ion (Li+) efflux in yeast cells is regulated by cellular energy levels and pH. Researchers propose an electrogenic proton/lithium ion (H+/Li+) antiport mechanism drives this efflux.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cuticle and pore plug properties in the table egg.

    Poultry science·2018
    Same author

    Unilateral blindness after orthognathic surgery: hypotensive anaesthesia is not the primary cause.

    International journal of oral and maxillofacial surgery·2017
    Same author

    Influence of the microstructure on the shell strength of eggs laid by hens of different ages.

    British poultry science·2002
    Same author

    Comparative functional features of plant potassium HvHAK1 and HvHAK2 transporters.

    The Journal of biological chemistry·2001
    Same author

    Ion homeostasis during salt stress in plants.

    Current opinion in cell biology·2001
    Same author

    Geometrical and crystallographic constraints determine the self-organization of shell microstructures in Unionidae (Bivalvia: Mollusca).

    Proceedings. Biological sciences·2001

    Area of Science:

    • Cellular Biology
    • Biochemistry
    • Microbiology

    Background:

    • Lithium ions (Li+) are utilized in various applications, necessitating an understanding of their cellular transport mechanisms.
    • Yeast (Saccharomyces cerevisiae) serves as a model organism for studying fundamental cellular processes, including ion transport.

    Purpose of the Study:

    • To elucidate the mechanism of lithium ion (Li+) efflux in yeast.
    • To investigate the factors influencing Li+ efflux, such as protonmotive force, ATP content, and intracellular pH.

    Main Methods:

    • Measuring Li+ efflux under varying conditions of protonmotive force and ATP levels.
    • Assessing the sensitivity of Li+ efflux to changes in intracellular pH.
    • Proposing a transport mechanism based on experimental observations.

    Main Results:

    • Yeast Li+ efflux is dependent on the protonmotive force.
    • Cellular ATP content regulates the rate of Li+ efflux.
    • Li+ efflux is sensitive to a decrease in intracellular pH.

    Conclusions:

    • An electrogenic proton/lithium ion (H+/Li+) antiport system is proposed as the primary mechanism for Li+ efflux in yeast.
    • This mechanism highlights the interplay between cellular energy status, pH homeostasis, and ion transport.

    Related Experiment Videos