The determination of choline in human semen by the enzymic method

Studies are reported on the specific enzymic microdetermination of free choline in human semen. The concentration of choline in normal semen was found to be 0.9 - 1.4 mg/ml. The minimum detectable concentration of choline using the method described was 1.5 micrograms. Normal levels of choline in vaginal fluid, saliva, serum and urine could not be detected by this procedure.