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[Production of a macrophage migration suppression factor when T-cells react with mutant H-2 antigens in mixed
The ability of T-cells producing the migration inhibition factor (MIF) to recognize point mutations of the K-end of the H-2 complex was revealed in a primary mixed lymphocyte culture. Nonimmune C57BL/6Y (H-2bm) lymphocytes produce MIF in response to the H-2 antigens of B6..C-G (zI)/Y (H-2bm1) and B6.M505/Y (H-2bm3) mutant strains. Similarly lymphocytes of the mutant strains react to the H-2b wild-type and reciprocally to each other. T-cell dependence of these reactions was shown by their abolition after pretreatment of responder cells with Thy-1.2 antiserum in the presence of complement. The ability of MIF-producing cells to recognize serologically unrecognizable H-2bm1 and H-2bm3 mutant antigens demonstrates high immunologic specificity and sensitivity of the MIF-reaction.
The ability of T-cells producing the migration inhibition factor (MIF) to recognize point mutations of the K-end of the H-2 complex was revealed in a primary mixed lymphocyte culture. Nonimmune C57BL/6Y (H-2bm) lymphocytes produce MIF in response to the H-2 antigens of B6..C-G (zI)/Y (H-2bm1) and B6.M505/Y (H-2bm3) mutant strains. Similarly lymphocytes of the mutant strains react to the H-2b wild-type and reciprocally to each other. T-cell dependence of these reactions was shown by their abolition after pretreatment of responder cells with Thy-1.2 antiserum in the presence of complement. The ability of MIF-producing cells to recognize serologically unrecognizable H-2bm1 and H-2bm3 mutant antigens demonstrates high immunologic specificity and sensitivity of the MIF-reaction.