Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Coding capacity of complementary DNA strands

A Casino, M Cipollaro, A M Guerrini

    Nucleic Acids Research
    |March 25, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Leopoldia comosa prevents metabolic disorders in rats with high-fat diet-induced obesity.

    European journal of nutrition·2018
    Same author

    Anther-specific expression of the rolB gene of Agrobacterium rhizogenes increases IAA content in anthers and alters anther development and whole flower growth.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same author

    Insulin-like growth factor binding proteins 4 and 7 released by senescent cells promote premature senescence in mesenchymal stem cells.

    Cell death & disease·2013
    Same author

    Efficient cultivation of neural stem cells with controlled delivery of FGF-2.

    Stem cell research·2012
    Same author

    Morphological and molecular characterization of healthy human ascending aorta.

    Histology and histopathology·2011
    Same author

    Genetic detoxification of bacterial toxins.

    Methods in molecular medicine·2011
    Same journal

    Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

    Nucleic acids research·2026
    Same journal

    Correction to 'Differentiable partition function calculation for RNA'.

    Nucleic acids research·2026
    Same journal

    Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

    Nucleic acids research·2026
    Same journal

    Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

    Nucleic acids research·2026
    Same journal

    Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

    Nucleic acids research·2026
    Same journal

    Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

    Nucleic acids research·2026
    See all related articles

    Computer analysis reveals longer open reading frames, termed "virtual genes," are more common on the complementary DNA strand than the coding strand. This suggests eukaryotic DNA strands may not be distinguishable for coding potential.

    Area of Science:

    • Genomics
    • Bioinformatics
    • Molecular Biology

    Background:

    • Traditional gene analysis focuses on coding DNA strands.
    • The functional potential of complementary DNA strands is less understood.
    • Open reading frames (ORFs) are key indicators of protein-coding potential.

    Purpose of the Study:

    • To investigate the distribution and significance of ORFs on both coding and complementary DNA strands.
    • To determine if eukaryotic structural genes can be distinguished based on their DNA strands.
    • To explore the potential coding capacity of complementary DNA strands.

    Main Methods:

    • Utilized a Fortran computer algorithm for nucleotide sequence analysis.
    • Analyzed both coding and complementary DNA strands of several eukaryotic structural genes.

    Related Experiment Videos

  • Examined intron sequences, splicing points, signal sequences, and gene mutations for "virtual genes" (ORFs > 100 codons).

    • Main Results:

    • ORFs shorter than 100 codons are randomly distributed on both DNA strands.
    • ORFs longer than 100 codons ("virtual genes") are significantly more frequent on the complementary DNA strand.
    • Analysis of structural features did not distinguish between coding and complementary strands for eukaryotic genes.

    Conclusions:

    • Eukaryotic structural genes cannot be reliably distinguished by their coding versus complementary DNA strands.
    • The complementary DNA strand of genes, such as human epsilon-globin, may possess coding potential for proteins.
    • This challenges conventional views of DNA strand usage in gene expression.