Actin filament bundles in vaccinia virus infected fibroblasts

When monolayer cultures of mouse 3T3 cells were infected with vaccinia virus, a rapid decrease of microfilament bundles was observed. The major disappearance of the cellular microfilaments occurred within the first hour after vaccinia virus infection, which is the period of uncoating of the virus core and early viral protein synthesis. The microfilament bundles of infected cells were significantly preserved in the presence of puromycin which inhibits viral protein synthesis.