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Related Experiment Videos

A rapid colorimetric assay for dry weight

A Bernal, J D Méndez, A Rosado

    Archivos De Investigacion Medica
    |January 1, 1981
    PubMed
    Summary

    This study introduces a rapid colorimetric method for determining dry weight in biological samples. The dichromate method offers greater sensitivity and precision than traditional techniques, making it ideal for various applications.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Biotechnology

    Background:

    • Accurate dry weight determination is crucial for biological and chemical analyses.
    • Traditional gravimetric methods can be time-consuming and less sensitive.

    Purpose of the Study:

    • To develop and validate a rapid, precise colorimetric method for dry weight determination.
    • To compare the dichromate method with standard gravimetric techniques.

    Main Methods:

    • Biological samples reacted with an acid dichromate solution at 92°C for 20 minutes.
    • Optical density of the reaction product measured at 630 nm.
    • Method validated for dry weight determination in the 100-1,000 microgram range.

    Main Results:

    • The dichromate method provides rapid (20 min) and precise dry weight measurements.
    • Demonstrated higher sensitivity and exactitude compared to standard weight methods.
    • The method is insensitive to inorganic compounds, suitable for tissue homogenates.

    Conclusions:

    • The dichromate colorimetric method is a superior alternative for dry weight determination.
    • Offers significant advantages in speed, sensitivity, and applicability to complex biological matrices.

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