Chicken buffy coat leucocytes (BCL) as indicator cells for human leucocytes migration inhibitory factor (LIF)

Human leucocyte migration inhibiting factor (LIF) form Con A stimulated lymphoid cells inhibited the migration of human and chicken peripheral blood leucocytes. The evaluation of human LIF activity on chicken cells is easy to perform as a routine test and has the advantage that large quantities of indicator cells are readily available. The chicken cell LIF test can be performed in the absence of serum, so avoiding effects due to plasminogen activator, and does not require the use of concentrated supernatants. LIF activity against both human and chicken targets was absent in supernatants from lung cancer subjects.