Synthesis of 3'-half molecule (nucleotides 36-76) of yeast alanine transfer ribonucleic acid

We have successfully ligated the 12 nucleotide fragment (nucleotides 46-57) with the p19p nucleotide fragment (nucleotides 58-76) to form a 31p nucleotide fragment (nucleotides 46-76) which after 5'-phosphorylation was in turn joined to the 10 nucleotide fragment (nucleotides 36-45) to give the 3'-half molecule of yeast alanine tRNA.