Monocyte-mediated antibody-dependent cytotoxicity. Modulation by Glycolysis and insulin

In suspensions of purified human monocytes from 14 healthy persons the antibody-dependent cell-mediated cytotoxicity (ADCC), the lactate release, and the glucose uptake were studied. In non-stimulated monocytes ADCC correlated with lactate release and glucose uptake. Following addition of insulin a dose-related rise in ADCC, lactate release, and glucose uptake was observed. For each of the three processes the maximal insulin effect was about 30%. Most of the stimulation was seen within the physiological concentration range of insulin, and the insulin concentration resulting in 50% of the maximal effect was nearly the same for ADCC, lactate release, and glucose uptake (about 100 pM). The insulin stimulation of ADCC correlated with the stimulation of lactate release and glucose uptake. An inverse correlation between the ADCC of non-stimulated monocytes and the insulin stimulation of ADCC was demonstrated. No relationship was found between monocyte maturity and any of the three variables of monocyte function, either with or without insulin. These results indicate that, for normal monocytes, the cytotoxic capacity is closely related to the glycolysis.