Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A design for a real-time fluorescence polarization computer

S S Stewart, R G Miller, G B Price

    Cytometry
    |November 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    After-Results of Gassing and Gunshot-wound Chest, especially in Relation to Tuberculosis.

    Postgraduate medical journal·2011
    Same author

    14-3-3s are DNA-replication proteins.

    Biochemical Society transactions·2002
    Same author

    Palliative care in amyotrophic lateral sclerosis.

    Neurologic clinics·2002
    Same author

    A central role for IL-2 in fate determination of mature T cells--I: role in determining the Th1/Th2 profile in primary T cell cultures.

    International immunology·2001
    Same author

    In vivo association of Ku with mammalian origins of DNA replication.

    Molecular biology of the cell·2001
    Same author

    Riluzole for amyotrophic lateral sclerosis (ALS)/motor neuron disease (MND).

    The Cochrane database of systematic reviews·2001

    We developed new hardware for flow cytometer cell sorters to calculate fluorescence polarization (FP) in real-time. This enables cell sorting based on FP measurements, advancing cell analysis capabilities.

    Area of Science:

    • Biophysics
    • Cell Biology
    • Instrumentation

    Background:

    • Flow cytometry is crucial for cell analysis.
    • Real-time fluorescence polarization (FP) measurements can enhance cell sorting.
    • Existing methods may lack real-time FP calculation capabilities.

    Purpose of the Study:

    • To develop and evaluate hardware components for real-time fluorescence polarization (FP) measurements in flow cytometry.
    • To enable cell sorting based on FP data on a cell-by-cell basis.
    • To assess the dynamic range of the developed system.

    Main Methods:

    • Developed novel hardware components for a flow cytometer cell sorter.
    • Implemented real-time calculation of fluorescence polarization (FP) or anisotropy.

    Related Experiment Videos

  • Utilized artificial electronic pulses to evaluate the system's dynamic range.
  • Performed real FP measurements on human peripheral blood lymphocytes labeled with fluorescein diacetate.

    • Main Results:

    • The developed hardware enables real-time, cell-by-cell FP calculations.
    • The system provides gating signals for sorting cells into two populations based on FP.
    • Artificial measurements confirmed the computer's useful dynamic range over a 40dB range.
    • Real FP measurements on lymphocytes were successfully obtained for comparison.

    Conclusions:

    • The new hardware components significantly enhance flow cytometry capabilities.
    • Real-time FP measurement and cell sorting are now feasible.
    • This advancement facilitates more precise cell population analysis and isolation.