The behaviour of embryonic chick and quail tissues in culture

Pieces of tissue were dissected from early chick and quail embryos (Stages XIII and XIV of Eyal-Giladi & Kochav, 1976; and stages 3-5 of Hamburger & Hamilton, 1951). These tissues were taken from three different regions of the early embryos, and from eight different regions of the older ones, and were derived mainly from the lower layer. Epiblast tissues were also used. The experiments were designed to test the ability of one tissue to penetrate another. A single tissue was grown in culture in a Falcon dish for 18-24 h until it had formed a coherent sheet of cells (Explant I). A second tissue was then combined with it in one of two ways: (a) A small piece of tissue (Explant II) was explanted on top of Explant I. In most cases Explant II penetrated through Explant I and spread on the Falcon dish. (b) Another small piece of tissue (Explant III) was explanted beside (in confrontation with) Explant I. Usually, Explant III penetrated into Explant I rather than vice versa. The results were analysed to see if there were any variations in behaviour of the different tissues. The main result was that important differences were found to exist between certain types of chick and quail cells when grown in culture; the implications of this finding for the widely used technique of xenoplastic grafting are mentioned. Another result was that Explant I was more likely to be penetrated when the second tissue was placed on top of it (Explant II) than when it was confronted with it (Explant III). The significance of these results is discussed.