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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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[Histamine-liberating action of MCD-peptide from bee venom]

I S Gushchin, A I Miroshnikov, V I Martynov

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |July 1, 1977
    PubMed

    Insights

    Histamine release from rat mast cells induced by MCD-peptide shares characteristics with compound 48/80. This process requires energy but is not dependent on cyclic adenosine-3

    Area of Science:

    • Immunology
    • Pharmacology
    • Cell Biology

    Background:

    • Mast cells are key immune cells involved in allergic reactions.
    • Histamine release is a critical mediator in inflammatory and allergic responses.
    • Compound 48/80 is a well-established agent for inducing mast cell degranulation.

    Purpose of the Study:

    • To investigate and compare histamine release induced by MCD-peptide from rat mast cells.
    • To characterize the mechanism of MCD-peptide-induced histamine release.
    • To compare MCD-peptide-induced histamine release with that induced by compound 48/80 and mellitin.

    Main Methods:

    • Isolated rat mast cells were used for in vitro experiments.
    • Histamine release was induced using MCD-peptide, mellitin, and compound 48/80.
    • Dose-response curves, latent period, temperature dependence, and energy dependence were analyzed.

    Main Results:

    • MCD-peptide induced histamine release with similar dose-response curves, latent periods, and temperature dependence as compound 48/80.
    • Histamine release induced by MCD-peptide is an energy-dependent process.
    • The process was found to be independent of cyclic adenosine-3',5'-monophosphate.

    Conclusions:

    • MCD-peptide is a potent inducer of histamine release from rat mast cells.
    • The mechanism of MCD-peptide-induced histamine release shares similarities with compound 48/80.
    • MCD-peptide-induced histamine release is an active, energy-requiring pathway.

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