Immunocytochemical localization of intermediate filament proteins during lymphocyte capping

Using double immuno-fluorescence techniques on frozen-thick sections, we have examined the fate of intermediate filaments during Con A receptor capping in lymphoid cells. Our results indicate that during capping intermediate filaments are preferentially aggregated between the surface receptor cap structure and the cell nucleus. It is possible, therefore, that intermediate filaments are directly involved in lymphocyte capping.