Related Experiment Videos
Initiation of chromosomal DNA synthesis in vitro
Molecular & General Genetics : MGG
|January 1, 1981
Abstract:
An in vitro complementation assay for initiation of chromosomal DNA replication is described. The initiation reaction is dependent upon extract from either of two hybrid-plasmid containing strains. Each hybrid plasmid carries a suppressor of dna A-ts mutations. The in vitro DNA synthesis is heavily biased toward the origin region, and the origin of replication (oriC) is replicated as determined by DNA-DNA hybridizations.