[Separation of proteolytic enzymes of polymorphonuclear leukocytes of pig peripheral blood]

Application of gel filtration through Sephadex G-100 (pH 7,5) and ion-exchange chromatography on KM-Sephadex G-50 (pH 7.2) made it possible to detect high-active proteolytic enzymes: elastase-like esterases and serine proteinases in the granular fraction of polymorphonuclear leucocytes of pig peripheral blood. Their pH optimum is for hydrolysis of p-butyltetroxy-L-alanine n-nitrophenyl ester in the weak alkaline medium, for the splitting of histones-in the neutral and weakly alkaline media. Ion-exchange chromatography revealed a sharp increase in the total activity of elastase-like esterase, which may evidence for a separation of the inhibitor at the given stage of the enzyme purification. This is also confirmed by a considerable decrease in the activity of the serine proteinase fraction when adding proteins possessing the activity of elastase-like esterase.