Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

32P-labeling test for DNA damage

K Randerath, M V Reddy, R C Gupta

    Proceedings of the National Academy of Sciences of the United States of America
    |October 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A Cross-Sectional Study on Patient Preferences for Selecting Surgeons for Joint Replacement Surgery in India.

    Cureus·2024
    Same author

    Decompression of Paralabral Cyst near Axillary Nerve: A Case Report.

    Journal of orthopaedic case reports·2023
    Same author

    Arthroscopic Anterior Capsule Reconstruction Using Fascia Lata Autograft and Knotless Fibretak: A Case Report.

    Journal of orthopaedic case reports·2023
    Same author

    Growth Mechanism of Micro/Nano Metal Dendrites and Cumulative Strategies for Countering Its Impacts in Metal Ion Batteries: A Review.

    Nanomaterials (Basel, Switzerland)·2021
    Same author

    Cause and Mitigation of Lithium-Ion Battery Failure-A Review.

    Materials (Basel, Switzerland)·2021
    Same author

    Custom 3D Printed Jigs in Salvage Reverse Shoulder Arthroplasty for Failed Four-Part Proximal Humerus Fracture Fixation: A Case Report.

    Journal of orthopaedic case reports·2020
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    This study introduces a 32P-labeling test to detect DNA adducts from chemical carcinogens. The method identifies covalent DNA binding, aiding in screening chemicals for mutagenic potential.

    Area of Science:

    • Molecular Biology
    • Toxicology
    • Biochemistry

    Background:

    • Chemical carcinogens and mutagens can form covalent adducts with DNA.
    • Detecting these DNA adducts is crucial for understanding chemical toxicity and mutagenicity.

    Purpose of the Study:

    • To develop and validate a sensitive method for detecting DNA adducts formed by covalent binding of chemicals.
    • To establish a screening test for chemicals capable of damaging DNA.

    Main Methods:

    • DNA samples treated with various known DNA-binding chemicals.
    • Enzymatic digestion of DNA to deoxynucleoside 3'-monophosphates.
    • 32P-labeling of monophosphates using T4 polynucleotide kinase and [gamma-32P]ATP.
    • High-resolution separation of labeled nucleotides using polyethyleneimine-cellulose thin-layer chromatography.

    Related Experiment Videos

    Main Results:

    • The 32P-labeling test successfully detected DNA adducts from multiple tested chemicals.
    • Each chemical produced a unique chromatographic spot pattern, allowing for specific identification.
    • The method demonstrated high sensitivity, detecting as little as one adduct per 10^5 DNA nucleotides.
    • The assay does not require the test chemical to be radiolabeled.

    Conclusions:

    • The 32P-labeling assay is a sensitive and specific method for detecting DNA covalent adducts.
    • This technique provides a valuable tool for screening chemicals for their DNA-damaging potential.
    • The method facilitates the identification of mutagenic and carcinogenic chemical agents.