Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laboratory variables in determining lecithin/sphingomyelin ratios

S L Parker

    The American Journal of Medical Technology
    |November 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Post-operative infection after minimally invasive versus open transforaminal lumbar interbody fusion (TLIF): literature review and cost analysis.

    Minimally invasive neurosurgery : MIN·2011
    Same author

    Non-specific binding and general cross-reactivity of Y receptor agonists are correlated and should importantly depend on their acidic sectors.

    Peptides·2010
    Same author

    Analysis of Maternal Deaths and Hospital Obstetrical Statistics in New York County.

    Bulletin of the New York Academy of Medicine·2009
    Same author

    Oligomerization of the heptahelical G protein coupling receptors: a case for association using transmembrane helices.

    Mini reviews in medicinal chemistry·2009
    Same author

    Dimers of the neuropeptide Y (NPY) Y2 receptor show asymmetry in agonist affinity and association with G proteins.

    Journal of receptor and signal transduction research·2008
    Same author

    Neuropeptide Y (NPY) Y2 receptors of rabbit kidney cortex are largely dimeric.

    Regulatory peptides·2008

    Amniotic fluid lecithin/sphingomyelin ratios are influenced by various testing methods. Controlling variables like sample contamination and quantification techniques is crucial for accurate fetal lung maturity assessment.

    Area of Science:

    • Biochemistry
    • Neonatal Medicine
    • Clinical Chemistry

    Background:

    • The lecithin/sphingomyelin (L/S) ratio in amniotic fluid is a key indicator of fetal lung maturity.
    • Accurate assessment of fetal lung maturity is critical for managing high-risk pregnancies and optimizing delivery timing.

    Purpose of the Study:

    • To highlight the method-dependent nature of amniotic fluid L/S ratio assays.
    • To identify key variables that impact the reliability of L/S ratio testing.

    Main Methods:

    • Thin-layer chromatography (TLC) is a common method for separating and quantifying amniotic fluid phospholipids.
    • Assay variables include sample contamination (blood, meconium), centrifugation, visualization reagents, and quantitation techniques.

    Main Results:

    Related Experiment Videos

    • Amniotic fluid L/S ratio results are significantly affected by procedural variables.
    • Inconsistent methodology leads to unreliable and potentially misleading assessments of fetal lung maturity.

    Conclusions:

    • Standardization of assay procedures is essential for reproducible and clinically useful L/S ratio results.
    • Careful control over sample handling and analytical techniques is necessary to ensure the accuracy of fetal lung maturity testing.