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Glomerular basement membrane: its antigenicity evaluated by antistreptococcal cell membrane antisera
Renal Physiology
|January 1, 1980
Summary
Rabbit antisera cross-react with glomerular basement membrane (GBM), showing age-dependent antigenicity. Specific antisera identified GBM antigens, aiding in their recognition for research and diagnostics.
Area of Science:
- Immunology
- Nephrology
- Biochemistry
Background:
- Rabbit antistreptococcal membrane antisera exhibit cross-reactivity with mammalian glomerular basement membranes (GBM).
- Previous studies indicated potential for broad anti-mammalian tissue reactivity, necessitating identification of GBM-specific antibodies.
Purpose of the Study:
- To characterize the specificity of rabbit antistreptococcal membrane antisera against human and mouse GBM.
- To investigate age-related changes in GBM antigenicity using these antisera.
Main Methods:
- Indirect fluorescent antibody testing (in vitro) on young and adult GBM tissue.
- In vivo testing of antisera activity in animals of different age groups.
Main Results:
- Selected antisera demonstrated specificity for GBM, distinguishing them from broadly reactive antibodies.
- GBM antigenicity showed significant age-dependent alterations, with distinct reactivity patterns observed in young (10-20 days) and adult (3 months) tissues.
- In vitro testing revealed higher reactivity with young GBM, while in vivo testing showed greater activity in older animals.
Conclusions:
- These specific antisera are valuable tools for identifying and characterizing GBM antigens.
- Understanding age-related changes in GBM antigenicity is crucial for accurate immunological studies and diagnostic applications.