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Updated: Aug 8, 2026

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
Published on: December 17, 2013
A novel extracellular proteinase from Bacillus pumilus
Abstract:
Bacillus pumilus NCTC AII6/73 produces a novel extracellular alkaline proteinase whose optimum is pH 9.5. The purified product from ion-exchange chromatography (DEAE-cellulose) was resolved into two bands by polyacrylamide-sodium dodecyl sulphate (SDS) gel electrophoresis. These had approximate molecular weights of 15.6 x 10(3) daltons, respectively, and a high content of glycine and proline, comprising roughly 50% of the total amino residues.
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