[Effect of exogenous acetyl group acceptors on cholinesterase biosynthesis in Arthrobacter simplex cells]

The presence of active acetyl or butyryl groups and their acceptors in the growth medium was found to be necessary for the high rate of cholinesterase biosynthesis in the cells of Arthrobacter simplex var. cholinesterasus. The active acetyl and butyryl groups are formed upon hydrolysis of acetylcholine and butyrylcholine as well as in the course of glucose metabolism. The following acids were shown to be the acceptors of the acetyl and butyryl groups: butyric, succinic, fumaric, malic acids and, to a less extent, alpha-ketoglutaric acid. The active acetyl and butyryl groups are bound with the acceptors under the control of coenzyme A in the reactions of fatty acid synthesis and the tricarboxylic acid cycle. Presumably, CoA regulates cholinesterase synthesis. The high rate of CoA binding in metabolic reactions provides conditions for the intensive synthesis of cholinesterase; the deceleration of these reactions inhibits the biosynthesis of cholinesterase.