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A method for recovering viruses from sludges

D Berman, G Berg, R S Safferman

Journal of Virological Methods

|December 1, 1981

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Treating sludge with divalent or trivalent cations and acidification effectively binds and elutes indigenous virions. This method improves viral recovery yields from wastewater sludge compared to simpler treatments.

Area of Science:

Environmental microbiology
Virology
Wastewater treatment

Background:

Wastewater sludge can harbor indigenous viruses.
Efficient methods are needed to recover these viruses for analysis.
Previous methods for viral recovery from sludge have limitations.

Purpose of the Study:

To develop and optimize a method for eluting indigenous virions from primary, activated, and anaerobic mesophilically digested sludges.
To compare the efficacy of combined salt and acid treatment with salt-only or acid-only treatments.

Main Methods:

Sludges were treated with magnesium chloride (MgCl2) or aluminum chloride (AlCl3) followed by acidification.
Adsorbed virions were eluted using buffered 10% beef extract.
Viral recovery yields were compared across different treatment combinations.

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Main Results:

The combined salt (divalent or trivalent cations) and acidification method significantly improved virion elution yields.
This combined treatment was superior to using salt or acidification alone.
Pre-homogenization of sludge did not enhance the number of recovered virions.

Conclusions:

A combined salt and acidification treatment followed by beef extract elution is an effective method for recovering indigenous virions from various types of sludge.
This optimized procedure enhances viral recovery efficiency in wastewater sludge analysis.