A simple one-step purification of human alpha 1-proteinase inhibitor by immunoadsorbent column chromatography

A one-step purification of human alpha 1-proteinase inhibitor was described using the rabbit anti-alpha 1-proteinase inhibitor antibody coupled to CNBr-activated Sepharose 4B. The elution of alpha 1-proteinase inhibitor from the immunoadsorbent column using 0.1 M Na2CO3/0.5 M NaCl solution gave an 85% yield. The properties of eluted alpha 1-proteinase inhibitor were identical with that of alpha 1-proteinase inhibitor that was purified by the conventional method. In addition, the specific activity of purified alpha 1-proteinase inhibitor was more than 93% of that of the theoretical value.