Related Experiment Video
Updated: Aug 10, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Published on: June 24, 2019
Elevated recombination and pairing structures during meiotic arrest in yeast of the nuclear division mutant cdc5
Abstract:
A diploid strain of yeast, homozygous for the mutation cdc5-1, undergoes a normal meiosis at 25 degrees C. At the nonpermissive temperature of 34 degrees C, meiosis is arrested at the first meiotic division, after premeiotic DNA replication and recombination commitment have taken place. Haploidisation commitment does not occur at 34 degrees C. Electron microscopy reveals that synaptons (synaptonemal complexes) are formed and the stage of arrest is characterised by a prevalence of "modified synaptons", which consist of paired lateral elements lacking the central elements. Prolonged incubation at this stage of arrest results in unusually high recombination levels, perhaps related to the synaptonal structures observed. Temperature shift-up experiments (transfers of cell from 25 degrees C to 34 degrees C at various times during meiosis) reveal that the CDC5 function is required for both the first and the second divisions of meiosis.
More Related Videos
Related Concept Videos
Nondisjunction
Separation of Sister Chromatids
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Meiosis II
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
Meiosis vs. Mitosis
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Nondisjunction

