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Related Experiment Videos

Multiple-puncture tuberculin testing: reason for variable response to tine test

C Herzog

    Tubercle
    |December 1, 1981
    PubMed
    Summary
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    Variations in tuberculin coating on Tine Test (TT) discs significantly impact results. Thick coatings lead to smaller induration sizes and more false negatives, limiting TT

    Area of Science:

    • Immunology
    • Microbiology
    • Public Health

    Background:

    • The Tine Test (TT) is a multipuncture device used for tuberculosis screening.
    • Variability in tuberculin antigen coating on TT discs has been suspected.
    • Standardization of diagnostic tests is crucial for reliable patient outcomes.

    Purpose of the Study:

    • To assess the variability of tuberculin coating on TT discs.
    • To evaluate the impact of coating thickness on TT test sensitivity.
    • To determine the clinical implications of TT coating variations.

    Main Methods:

    • Microscopic examination of 1040 TT discs from three production batches.
    • Quantification of tuberculin coating thickness (thin, medium, thick).
    • Comparison of induration sizes from thin vs. thick coated TT in 103 positive volunteers.

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    Main Results:

    • Significant variations in tuberculin coating thickness were observed across batches (p < 0.001).
    • Thickly coated TT discs resulted in substantially smaller induration diameters (p < 0.001).
    • Thick TT failed to detect 16% of positive subjects (≥2mm cutoff), compared to 5% for thin TT (p < 0.05).

    Conclusions:

    • Tuberculin coating thickness on TT discs is highly variable.
    • Thick coatings significantly reduce TT sensitivity, increasing false-negative results.
    • TT's value as a screening tool is limited without guaranteed uniform tuberculin coating.