[2-wave-length method for the quantitative determination of DNA and histones in the same cell nucleus]

A cytophotometric method is proposed for measuring DNA and histones in the same nucleus after two consecutive cytochemical reactions. Optimum conditions are defined for realization of the reactions. From spectral curves of components under investigation equations are concluded for calculations of DNA and histone contents, according to the data obtained from measuring the optical density of the nucleus at the two specially selected wave lengths. The method ensures receiving results with the same accuracy as ordinary methods of DNA and histone determination.