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Related Experiment Videos

Physical randomization of tissue architecture: an alternative to systemic sampling

B M Stringer, D Wynford-Thomas, E D Williams

    Journal of Microscopy
    |May 1, 1982
    PubMed
    Summary

    This study presents a reliable method for quantifying tissue morphology in rat thyroids. Dicing tissue before embedding ensures accurate morphometry even with physiological changes.

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    Area of Science:

    • Histology
    • Morphometry
    • Endocrinology

    Background:

    • Accurate quantitation of tissue morphology is crucial for understanding physiological states.
    • Traditional methods can be labor-intensive and require numerous sections.
    • Developing efficient morphometric techniques is essential for biological research.

    Purpose of the Study:

    • To develop and validate a rapid and accurate method for quantifying tissue morphology.
    • To assess the reliability of the method across different physiological conditions.
    • To reduce the number of sections required for morphometric analysis.

    Main Methods:

    • Rat thyroid tissue was diced and randomly embedded in epon.
    • Sections were cut at 500 micrometer intervals and stained with toluidine blue.

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  • Epithelial, follicular lumen, and stromal components were quantified using point counting, with variance analysis.

    • Main Results:

    • No significant variation in component distribution was observed between sections of normal diced thyroid.
    • The method remained reliable for diced thyroids from animals treated with goitrogen, indicating robustness.
    • Dicing significantly reduced the number of sections needed for accurate morphometry.

    Conclusions:

    • The described dicing and embedding method provides a reliable and efficient approach for tissue morphometry.
    • This technique is accurate even when significant changes in tissue component distribution occur.
    • The method enhances the accuracy and reduces the workload in morphometric studies of non-randomly distributed tissues.