Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cryptococcal capsular polysaccharide clearance in nonimmune mice

H G Muchmore, E N Scott, F G Felton

    Mycopathologia
    |April 23, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Human mycoses and current antifungal therapy.

    Drug news & perspectives·2004
    Same author

    Medical mycology and current trends in antifungal chemotherapy.

    Drug news & perspectives·2004
    Same author

    Advances in antifungal chemotherapy.

    Drug news & perspectives·2004
    Same author

    Progress in antibacterial and antifungal chemotherapy.

    Drug news & perspectives·2003
    Same author

    Human mycoses and advances in antifungal therapy.

    Drug news & perspectives·2003
    Same author

    Yeasts and fluconazole susceptibility in the Philippines.

    Mycopathologia·2000

    This study tracked cryptococcal polysaccharide (CP) clearance in mice. The liver and spleen showed the longest retention of CP, indicating significant storage capacity.

    Area of Science:

    • Immunology
    • Microbiology
    • Pharmacokinetics

    Background:

    • Cryptococcal polysaccharide (CP) is a key component of Cryptococcus fungal cell walls.
    • Understanding the clearance and tissue distribution of CP is crucial for managing cryptococcal infections.

    Purpose of the Study:

    • To investigate the clearance kinetics of cryptococcal polysaccharide (CP) from serum and tissues in nonimmune mice.
    • To determine the primary tissues responsible for CP storage and identify the duration of its detectability.

    Main Methods:

    • Intravenous injection of purified CP into nonimmune mice.
    • Collection of serum, urine, and tissue samples at various intervals up to 84 days.
    • Quantification of CP using enzyme-linked immunosorbent assay (ELISA) and latex agglutination.

    Related Experiment Videos

    Main Results:

    • High CP concentrations were detected in serum, liver, spleen, kidney, and lung shortly after injection.
    • CP was detectable for up to 70 days in the liver and spleen, but only 14 days in lung tissue.
    • The liver and spleen demonstrated the greatest capacity for CP storage, retaining detectable levels longer than other tissues.

    Conclusions:

    • Cryptococcal polysaccharide is stored in tissues, with the liver and spleen having a higher storage capacity.
    • The exact binding mechanism and site of CP storage remain unknown.
    • IgM antibody to CP appeared around day 14, suggesting an immune response initiation.