Regulation of acetylcholinesterase by nerve growth factor in the pheochromocytoma PC12 cell line

In the PC12 clonal nerve cell line derived from a rat pheochromocytoma, the activity of acetylcholinesterase (AChE) is regulated by nerve growth factor (NGF). The specific activity of AChE was significantly increased 2 days after exposure of PC12 cells to NGF (8 X 10(-8) M), and reached its maximal increase of 94% after 3 days. Thereafter the specific activity of AChE did not significantly vary during the following 7 days. AChE cytochemistry showed the enzyme activity to be localized predominantly in cisternae of the rough endoplasmic reticulum, with a strongly enhanced activity after NGF treatment.