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Related Experiment Videos

Ca++-induced cataract

K R Hightower, V N Reddy

    Investigative Ophthalmology & Visual Science
    |February 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Elevated calcium levels cause cataracts in rabbit lenses by binding to proteins and membranes. This bound calcium is difficult to remove, unlike calcium in soluble proteins.

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    Area of Science:

    • Ophthalmology
    • Biochemistry
    • Cell Biology

    Background:

    • Cataracts are a leading cause of vision impairment.
    • The role of intracellular calcium in cataract formation is not fully understood.
    • Previous studies suggest a link between calcium and lens opacification.

    Purpose of the Study:

    • To investigate the effect of elevated intracellular calcium on cultured rabbit lenses.
    • To determine the binding sites and characteristics of excess calcium within the lens.
    • To elucidate the mechanism of calcium-induced cataract formation.

    Main Methods:

    • Cultured rabbit lenses were treated to increase internal calcium levels.
    • Levels of total and bound calcium (Ca++) were measured.
    • Sodium, potassium, and water content were analyzed.

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  • Dialysis was used to assess calcium binding to different lens fractions.

    • Main Results:

    • Experimental procedures successfully increased total and bound Ca++ levels.
    • Calcium elevation often occurred without significant changes in sodium, potassium, or water content.
    • A significant portion of excess calcium bound to membranes and water-insoluble proteins, persisting after dialysis.
    • Calcium bound to water-soluble proteins was largely removed by dialysis.

    Conclusions:

    • Elevated intracellular calcium is a key factor in cataract formation in rabbit lenses.
    • Calcium binding to lens membranes and insoluble proteins contributes to cataract development.
    • The differential binding of calcium to lens components has implications for understanding cataract pathogenesis.