Purification of cytoplasmic aldehyde dehydrogenase by covalent chromatography on reduced thiopropyl-Sepharose 6B

Disulfiram-modified cytoplasmic aldehyde dehydrogenase binds covalently to the thiol groups of reduced thiopropyl-Sepharose 6B under conditions in which mitochondrial aldehyde dehydrogenase does not bind. After washing the resin, the uncontaminated form of the enzyme is eluted by dithiothreitol solution.