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Murine serum glycoprotein gp70 behaves as an acute phase reactant
The Journal of Experimental Medicine
|February 1, 1982
Summary
Bacterial lipopolysaccharide (LPS) induces glycoprotein gp70 in mouse serum, originating from liver cells. This response mirrors acute phase reactants (APR) and is independent of T and B cell activation.
Area of Science:
- Immunology
- Biochemistry
- Hepatology
Background:
- Glycoprotein gp70 is a host constituent.
- Bacterial lipopolysaccharide (LPS) is a potent immune stimulant.
Purpose of the Study:
- To investigate the source and regulation of serum glycoprotein gp70.
- To determine if gp70 is an acute phase reactant (APR).
Main Methods:
- Intraperitoneal injection of LPS and other APR inducers in mice.
- Serum analysis for gp70 levels.
- Serological and immunohistochemical studies for gp70 production.
- Assessment of T and B cell involvement.
Main Results:
- LPS injection rapidly increased serum gp70 levels in mice.
- gp70 production was localized to hepatic parenchymal cells.
- The gp70 response was independent of T and B cell activation.
- Serum gp70 kinetics mimicked those of known APRs.
Conclusions:
- The liver is the primary source of serum gp70.
- Serum gp70 functions as an acute phase reactant (APR).
- gp70 expression is regulated similarly to other APRs.
- gp70 is a normal host protein, not of viral origin.