Detoxification of aflatoxin contaminated corn by methanol extraction
Related Experiment Video
Updated: Oct 1, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
Abstract:
Corn containing 500 ppb aflatoxin was decontaminated by methanolic extraction. Ninety percent of the aflatoxin was removed in one three-minute blending with a solvent to corn ratio of 4:1. Recycling methanol for additional extractions could be done by removal of aflatoxin by carbon or distillation of the methanol. Methanol costs per bushel of corn without recycling would probably be expensive. By recycling, the cost is estimated to be ten to twenty cents per bushel in a laboratory size experiment. A source of ethanol on the farm could make the process more feasible. Chicks fed decontaminated corn showed no signs of aflatoxin toxicity. Fatty degenerated livers were produced in chicks fed the nondetoxified corn. Other mycotoxins which are not affected by the ammoniation process used to decontaminate corn, can be removed by this process.
More Related Videos
09:44RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
Published on: December 21, 2015
09:21Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
Published on: February 15, 2019