Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A long-lived thymidine pool in epithelial stem cells

W J Hume, C S Potten

    Cell and Tissue Kinetics
    |January 1, 1982
    PubMed
    Summary

    Mouse tongue filiform papillae stem cells exhibit a delayed DNA synthesis response after [3H]TdR injection. This suggests a long-lived precursor pool for DNA synthesis in these cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The stem cells of small intestinal crypts: where are they?

    Cell proliferation·2009
    Same author

    Epithelial stem cell-related alterations in Trichinella spiralis-infected small intestine.

    Cell proliferation·2009
    Same author

    Adult small intestinal stem cells: identification, location, characteristics, and clinical applications.

    Ernst Schering Research Foundation workshop·2006
    Same author

    Regional localisation of p53-independent apoptosis determines toxicity to 5-fluorouracil and pyrrolidinedithiocarbamate in the murine gut.

    British journal of cancer·2006
    Same author

    Teduglutide ([Gly2]GLP-2) protects small intestinal stem cells from radiation damage.

    Cell proliferation·2004
    Same author

    Acromegaly and colorectal cancer: a comprehensive review of epidemiology, biological mechanisms, and clinical implications.

    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2004

    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Oral Biology

    Background:

    • The circadian rhythm influences cell proliferation in various tissues.
    • Understanding stem cell dynamics is crucial for tissue regeneration and development.
    • Filiform papillae of the tongue are constantly renewing structures with a defined basal cell population.

    Purpose of the Study:

    • To investigate the cell cycle kinetics of basal cells in mouse tongue filiform papillae.
    • To identify potential long-lived precursor pools for DNA synthesis in these cells.
    • To explore the influence of circadian rhythms on DNA labeling index.

    Main Methods:

    • Assessment of labeling index (LI) over time after [3H]TdR injection in mouse tongue filiform papillae.
    • Utilizing vincristine and hydroxyurea to investigate cell cycle progression and DNA synthesis.
    • Analysis of basal cell positions within the anterior column of filiform papillae.

    Main Results:

    • An initial doubling of the labeling index in the stem cell zone was observed due to cell division.
    • A secondary rise of 14-16% in LI was detected 16 hours post-injection, even with vincristine.
    • Hydroxyurea largely prevented [3H]TdR uptake and initial LI doubling, but the secondary LI rise persisted.

    Conclusions:

    • A subset of stem cells in the filiform papillae may store [3H]TdR in a long-lived precursor pool for at least 16 hours.
    • This finding suggests selective DNA segregation at mitosis by stem cells.
    • The results complement previous work on stem cell behavior in this model system.

    Related Experiment Videos