[Circahoral fluctuations in protein release from hepatocytes in a monolayer culture]

An account is presented on morphological and functional properties in primary monolayer culture. Advantages of this model, as compared to the suspension cells, for studying various aspects of the liver vital activity are described. Dynamics of protein secretion by hepatocytes during a 2-hour period was investigated by different methods. The data obtained point to cyclic changes of the rate of protein secretion in the liver cells with circahoralian periodicity, which is typical of the changes in the rate of protein synthesis we revealed earlier for the same cells.