[Two-dimensional gel electrophoresis on nonhistone proteins of chromatin from rat liver and lung]

The proposed method for phenol-soluble nonhistone proteins (ph-NHP) analysis is based on a two-dimensional gel electrophoresis which can be conducted without phenol removal. For this reason some intermediate steps are excluded so that the experiment can be completed in a significantly less time and protein losses are minimal. The method permits a high resolution and well-reproducible results. The ph-NHP of rat liver and lung, when analysed with this method, were shown to be very heterogeneous. The total number of fractions for each organ was over 80. At the same time the differences detected in the patterns of the ph-NHP were mainly quantitative ones.