Sexual dimorphism in succinic dehydrogenase staining of toad pretrigeminal nucleus

The anuran (toad and frog) pretrigeminal nucleus is probably involved in the production of vocalizations, including the male mating call. This nucleus is especially obvious when stained by the succinic dehydrogenase method. In the American toad, a striking sexual dimorphism is shown by such staining. The male nucleus contains numerous, large cells that stain intensely. Such cells are smaller and infrequent in females.