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Inhibition of La Crosse virus replication by monensin, monovalent ionophore
The Journal of General Virology
|March 1, 1982
Abstract:
The effect of monensin, a monovalent ionophore, on La Crosse virus particle formation and polypeptide synthesis was examined. Monensin inhibited the release of virus particles (both infectious and non-infectious) from infected BHK-21 cells. Monensin had no detectable effect on the synthesis of polypeptides G1, G2, and N.
Insights
Monensin, an ionophore, blocks La Crosse virus release from cells but does not affect viral polypeptide synthesis. This suggests monensin interferes with virus particle assembly or egress.
Area of Science:
- Virology
- Cell Biology
- Drug Discovery
Background:
- La Crosse virus (LACV) is an important human pathogen.
- Understanding virus particle formation is crucial for developing antiviral strategies.
- Monensin is a known ionophore affecting cellular processes.
Purpose of the Study:
- To investigate the impact of monensin on La Crosse virus particle formation.
- To determine if monensin affects viral polypeptide synthesis.
Main Methods:
- Infection of BHK-21 cells with La Crosse virus.
- Treatment of infected cells with monensin.
- Quantification of infectious and non-infectious virus particles.
- Analysis of viral polypeptide synthesis (G1, G2, N).
Main Results:
- Monensin significantly inhibited the release of both infectious and non-infectious La Crosse virus particles from infected cells.
- Monensin did not affect the synthesis of key viral polypeptides: G1, G2, and N.
Conclusions:
- Monensin disrupts La Crosse virus particle release, likely affecting post-translational modification, assembly, or egress.
- The drug's effect is specific to virus release, not viral protein synthesis.