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Published on: May 9, 2014
Theoretical and mathematical conditions for use of linearization methods for saturation-type radioassay data
Abstract:
The algebraic equivalency of nine different methods for the linearization of saturation-type radioassay data was previously demonstrated mathematically to hold under the assumption that the antibody is saturated with ligand. Although compete saturation with antibody never occurs, a good approximation to a saturation condition occurs when the product of the labeled ligand and the affinity constant is 100 or more. The mathematical basis for determining when methods for the linearization of saturation-type radioassay data are valid is derived from a development of the first-order mass action law applied to ligand-antibody reactions.
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